ʻO ka hale mana ma lalo o ka honua anti-old - NMNH

01.NMNHhoʻonui i nā pae cellular NAD + a me NADH. Ua hoʻohālikelike nā mea noiʻi i nā hopena o NMN a me NMNH i ka hoʻonui ʻana i nā pae cellular NAD +. Hoʻonui ka mālama ʻana me NMNH (100 μM) i nā pae cellular NAD + e 5-fold i nā cell HepG2, ʻoiai ʻo 100 μM NMN i hoʻonui iki i nā pae NAD +. Ma waho aʻe o nā pūnaewele HepG2, ua hōʻoia pū nā mea noiʻi ua hoʻonui ʻo NMNH i ka NAD + i loko o nā pūnaewele ES-2 a me 3T3-L1, ʻo ia nā pūnaewele i loaʻa i ka ovary kanaka a me nā ʻiole embryonic fibroblast-derived cell, kēlā me kēia. A ua ʻike pū ka poʻe noiʻi ua hoʻonui ʻo NMNH i nā pae cellular NAD + i nā ʻāpana 786-O i kahi manawa a me ka hilinaʻi ʻana. Ua ana nā mea noiʻi i ka NADH me ka hoʻohana ʻana i ka spectrometry lehulehu a ʻike ʻia ʻo nā pae NADH i nā cell HepG2 i mālama ʻia e NMNH he 2.5 mau manawa kiʻekiʻe ma mua o nā cell i mālama ʻole ʻia a NMN, a ua ʻike pū ʻia nā pae kiʻekiʻe NADH i loko o nā cell 786-O i mālama ʻia e NMNH. Ua ʻike pū mākou he 340 mg/kg NMNH ka mālama ʻana i hoʻonui i nā pae NADH i loko o nā ate ʻiole e 3-fold, aʻo 340 mg/kg NMN hoʻonui i nā pae NADH e 1.7-fold.

Kiʻi 3.4. Hoʻonui ka NMNH i nā pae NMNH

02NMNH kaohi glycolysis a me TCA pōʻaiapuni. ʻO ka hōʻiliʻili ʻana o NADH e lohi i ka glycolysis a me ka pōʻai TCA. No ka nānā ʻana i ka pane metabolic o nā cell i ka mālama ʻana i ka NMNH, ua hana nā mea noiʻi i ka loiloi metabolomic oNMNH-a me nā pūnaewele i mālama ʻia e NMN. Ua ʻike ʻia ʻo ka mālama ʻana i ka NMNH ʻaʻole i hoʻemi wale i nā pae o ka glycolysis intermediates, akā ua hoʻemi pū ʻia nā pae o ka TCA cycle intermediates. Hōʻike kēia mau hopena i ka hōʻiliʻili ʻana o ka NADH i hana ʻia e NMNH e kāohi i ka glycolysis cellular a me ka pōʻai TCA.

Kiʻi 6. ʻIkepili Metabolomics

03.NMNHkeʻakeʻa i ka ulu ʻana o ke kelepona Ua ʻimi nā mea noiʻi i ka hopena o ka NMNH i ka ulu ʻana o ka cell a ʻike i ka mālama ʻana i ka NMNH i keʻakeʻa i ka ulu ʻana o ka cell HepG2 ma nā ʻano kiʻekiʻe ma mua o 250 μM. Ua kālailai nā mea noiʻi i ka pōʻaiapili pūnaewele ma nā pūnaewele HepG2 i mālamaʻia me 1 mM NMNH a iʻole NMN no nā hola 12 a uaʻikeʻia ua hoʻopaʻaʻia ka NMNH i ka pōʻaiapili pūnaewele,ʻoiaiʻaʻole i loaʻa ka hopena o NMN i ka pōʻai pūnaewele. Eia kekahi, uaʻike pū nā mea noiʻi ua pale loaʻo 500 μM NMNH i ka uluʻana o nā pūnaewele 786-O; Hiki i ka 250 μM NMNH ke kaohi pono i ka ulu ʻana o ke kelepona ma nā pūnaewele HK-2.

Kiʻi 7.8.NMNH keʻakeʻa i ka ulu ʻana o ka cell in vitro

04.NMNHUa hana nā mea noiʻi palekana i nā hoʻokolohua e pili ana i ka nui o ka nui o ka NMNH i ka hepatotoxicity. ʻO ka mālama ʻana i nā ʻiole kāne C57BL/6J me 50, 100, 500, a i ʻole 1000 mg/kg NMNH ma o ka intraperitoneal injection i kēlā me kēia lā ʻē aʻe no hoʻokahi pule i hōʻike ʻole ʻia ka emi ʻana o nā pae serum alanine aminotransferase (ALT) a me aspartate aminotransferase (AST). Hoʻokiʻekiʻe ʻia, e hōʻike ana ʻaʻole hepatotoxic ʻo NMNH i nā kau kiʻekiʻe. Eia kekahi, ua ʻike pū nā mea noiʻi ua hoʻonui ka mālama ʻana i ka NMNH i ka ʻike NAD + i loko o nā ʻiole i loko o ke ʻano i hilinaʻi ʻia. Hoʻonui ka mālama ʻana me 1000 mg/kg NMNH i nā pae NAD+ i loko o nā ʻiole ma mua o 10 mau manawa.

Helu 10.NMNHhoʻonui i ka pae o NAD + i loko o ke ake o nā ʻiole

Ua hōʻike nā haʻawina ʻo NMNH kahi mea hoʻohui NAD + -boosting ʻoi aku ka maikaʻi ma mua o ka NMN ma in vitro a me in vivo; Eia kekahi, hoʻonui ka NMNH i nā pae NAD (NADH) i hoʻemi ʻia i nā cell a me nā ate ʻiole. Ua hōʻike ka loiloi Metabolomic i ka NMNH ke kāohi i ka glycolysis a me ka pōʻai TCA. Hōʻike nā hoʻokolohua in vitro e hoʻoulu ʻo NMNH i ka hopu ʻana i ka pōʻai cell a keʻakeʻa i ka ulu ʻana o ka cell. Hoʻohui pū ʻia, hōʻike kēia haʻawina ʻo NMNH kahi mea hoʻonui NAD + ikaika a keʻakeʻa nei i ka glycolysis, TCA cycle, a me ka ulu ʻana o ke kelepona.

ʻO ka inoa Kina o NMNH he "reduced β-nicotinamide mononucleotide", ʻo ia ke ʻano hōʻemi o NMN a he pauka melemele amorphous. ʻO ke kumu molekala: C11H17N2O8P Ke kaumaha molekalale: 336.23g/molCAS No.: 108347-85-9 Hōʻike nā palapala noiʻi hou loa e hoʻonui ʻo NMNH i nā pae NAD+ me ka wikiwiki a ma kahi kiʻekiʻe kiʻekiʻe, ma ka awelika 5 mau manawa o ka NMN